Sit and biological control of anastrepha fruit flies in mexico. Functional response and superparasitism by diachasmimorpha. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer, from where it. Effects of host age and radiation dose in diachasmimorpha. Evaluation of volumes of ceratitis capitata larvae in. Diachasmimorpha longicaudata ashmead, a solitary koinobiont larvalprepupal endoparasitoid of fruit flies, was introduced into kenya for testing and final release against the recently discovered i. An diachasmimorpha longicaudata in uska species han hymenoptera nga syahan ginhulagway ni william harris ashmead hadton 1905.
Augmentative releases of diachasmimorpha longicaudata. Braconidae is a solitary species of parasitoid wasp and widely used in integrated pest management ipm programs as a biological control agent in order to suppress tephritid fruit flies of economic importance. Dispersal capacity of fruit fly parasitoid diachasmimorpha. Although many studies have investigated the behaviors in the detection of their hosts, little is known of the. The parasitic wasp, diachasmimorpha longicaudata ashmead hymenoptera. Fieldopen augmentative releases were conducted to assess the efficacy of diachasmimorpha longicaudata ashmead for the regulation of ceratitis capit. An asian opiine species, diachasmimorpha longicaudata ashmead has been introduced throughout much of the americas. Augmentative releases of diachasmimorpha longicaudata hymenoptera. Density of diachasmimorpha longicaudata ashmead and host. The larvalpupal endoparasitoid diachasmimorpha longicaudata hymenoptera. A virulent entomopathogenic nematode epn species was investigated to suppress the fruittosoil stages of caribflies, which are also attacked by the koinobiont parasitoid diachasmimorpha longicaudata in south florida. Diachasmimorpha longicaudata 865 multivariate analyses of variance manova p diachasmimorpha longicaudata hymenoptera. Braconidae is increasingly being used in integrated. Diachasmimorpha longicaudata parasitism response to.
It has been massreared and used in augmentative releases against major fruit fly species in tropical and subtropical countries, and recently in the mediterranean basin. Innate and acquired behavioral responsessuch as pest host fruit preferenceof parasitoids can modulate their efficiency in the field and. The ncs were 30 nm in diameter and nearly 900 nm in length. The preferences of diachasmimorpha longicaudata ashmead for larvae of anastrepha fraterculus wiedemann and ceratitis capitata wiedemann were evaluated under laboratory conditions in nochoice and dualchoice tests, based on percent parasitism, proportion of emerged parasitoids, proportion of female offspring, and number of parasitoid female visits to and ovipositor probes on the. The dlepv 250300 kb double stranded dna genome encodes putative proteins having 30 to 60% amino acid identity with poxvirus homologs such as dna. Pdf parasitism of diachasmimorpha longicaudata ashmead on. Diachasmimorpha longicaudata ashmead at bioplanta san juan massrearing facility 3. Diachasmimorpha longicaudata is a solitary species of parasitoid wasp and an endoparasitoid of tephritid fruit fly larvae.
The functional response and the effect of superparasitism of diachasmimorpha longicaudata ashmead in larvae of anastrepha ludens loew was determined under laboratory conditions. Parasitized puparia of both species were shipped to karc. Diachasmimorpha longicaudata originated from the indopacific region, has been widely disseminated into america via hawaii, and can be considered. Braconidae is increasingly being used in integrated pest management ipm programs as a biological control agent in order to suppress tephritid fruit flies of economic importance. Effect of insectary rearing on host preference and. The ectoparasitic pupal parasitoid, pachycrepoideus. Side effects of toxic bait formulations on diachasmimorpha. Sit and biological control of anastrepha fruit flies in mexico d. Characterization of a venom glandassociated rhabdovirus. Transcriptome profiling of diachasmimorpha longicaudata towards. Tephritidae, with the aim of finding a treatment that prevented adult fly emergence, yet did not adversely affect larval quality as rearing hosts for the parasitoid diachasmimorpha longicaudata ashmead hymenoptera. Abiotic factors affecting diachasmimorpha longicaudata. Ovipositor length in a guild of parasitoids hymenoptera. Responses of anastrepha suspensa, diachasmimorpha longicaudata, and sensitivity of guava production to heterorhabditis bacteriophora in fruit fly integrated pest management abstract caribbean fruit fly, also known as caribfly or anastrepha suspensa, is a major tephritid pest of guavas.
Braconidae massreared on medfly larvae of the tsl vienna 8 genetic sexing strain. Innate host habitat preference in the parasitoid diachasmimorpha. Effects of host age and radiation dose in diachasmimorpha longicaudata hymenoptera. Larger fruits may provide more nutrients, produce larger host flies and in turn, affect the parasitoid size. Braconidae is commonly used for both classical and augmentative release in programs designed to control tephritid fruit flies clausen, 1978, purcell, 1998, ovruski et al.
This is the first facility in argentina to produce d. Insects free fulltext diachasmimorpha longicaudata. The fore and hindwings of an adult utetes anastrephae viereck, a wasp parasitoid of anastrepha spp. Superparasitism in the fruit fly parasitoid diachasmimorpha longicaudata hymenoptera. Ecology opulation biology ovipositor length in a guild. Pdf responses of anastrepha suspensa, diachasmimorpha. Larger female parasitoids produce more offspring than smaller females. The braconid diachasmimorpha longicaudata is the most widely used parasitoid in biological control programs of tephritids. This is illustrated by a recent study in key biscayneandclewiston,florida,usingthebraconidlarval pupal parasitoid d.
Dispersion patterns of diachasmimorpha longicaudata hymenoptera. Photograph by charles stuhl, usdaarscmave gainesville, florida. Adult parasitoids were tested individually and in groups of 5. The study of native anastrepha parasitoids has a long history and began in mexico. Diachasmimorpha longicaudata is an old world parasitoid of tephritid fruit flies that was widely introduced in the americas to control pest species. A new rodshaped virus from parasitic wasp diachasmimorpha. Florida entomologist contains contributions on all aspects of basic and applied entomological science from all geographic regions. Pickett4 1division of insect biology and center for biological control, university of california, berkeley. We evaluated the effects of xray irradiation on larvae of ceratitis capitata wiedemann diptera.
The main objective was to develop a feasible and cost. Abstract diachasmimorpha longicaudata ashmead hymenoptera. Seasonal occurrence of diachasmimorpha longicaudata. Evaluation of the impact of diachasmimorpha longicaudata. Effect of density and lethargy duration in prerelease. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. Braconidae is a solitary koinobiont larvalprepupal endoparasitoid of tephritid species 1114. After the releases of diachasmimorpha longicaudata. Effects of releasing two diachasmimorpha longicaudata. A homolog of the vaccinia virus dl rifampicin resistance gene is in the entomopoxvirus of the parasitic wasp, diachasmimorpha longicaudata. Different densities prerelease packing and times of lethargy in the fruit fly parasitoids diachasmimorpha longicaudata ashmead were evaluated in order to standardize the process of chilled insect technique for this species.
An diachasmimorpha longicaudata in nahilalakip ha genus nga diachasmimorpha, ngan familia nga braconidae. Diachasmimorpha longicaudata 195 floral food sources flower morphology and parasitoid survival. B c p intrinsic competition and competitorfreespace. Effects of releasing two diachasmimorpha longicaudata population lines for the control of ceratitis capitata infesting three key host fruit species.
Spatial dynamics of two oriental fruit fly diptera. Life history parameters of diachasmimorpha longicaudata on. Braconidae is currently the most commonly employed biological control agent against tephritid fruit flies in the americas. Research article open access transcriptome profiling of diachasmimorpha longicaudata towards useful molecular tools for population management m. Responses of anastrepha suspensa, diachasmimorpha longicaudata, and sensitivity of guava production to heterorhabditis bacteriophora in fruit fly integrated pest management. Braconidae is considered one of the main biological control agents of ceratitis capitata wiedemann diptera. Rearing of the fruit fly parasitoid diachasmimorpha. A homolog of the vaccinia virus dl rifampicin resistance. Diachasmimorpha longicaudata ashmead is a koinobiont endoparasitoid of tephritidae larvae. Diachasmimorpha longicaudata ashmead, 1905 hymenoptera.
As a result of this production, currently 200,000 parasitoid adults are produced weekly in the bioplanta san juan facility using c. Diachasmimorpha is a genus of the opiinae subfamily of insects and was first described in 19. Pdf life history parameters of diachasmimorpha longicaudata on. Insects free fulltext diachasmimorpha longicaudata parasitism. Transcriptome profiling of diachasmimorpha longicaudata. The cu2 submarginal cell of the forewing is fivesided and relatively long compared to diachasmimorpha longicaudata and doryctobracon areolatus wharton and marsh 1978. It is considered among the successful parasitoid species currently used in bc programs, despite the controversy of its species status 11,12. Fopius arisanus was the dominant parasitoid at all three elevations, diachasmimorpha longicaudata ashmead occurred consistently, and t. The value of this parasitoid derives from its efficacy of parasitism and ability to. Pdf effect of diachasmimorpha longicaudata releases on. Diachasmimorpha formerly biosteres or opius longicaudata ashmead wharton 1987 is a solitary braconid wasp parasitoid of caribbean fruit fly, anastrepha suspensa loew, larvae. Superparasitism in mass reared diachasmimorpha longicaudata ashmeadhymenoptera. Hymenopterous larvalpupal and pupal parasitoids of. The parasitoid diachasmimorpha longicaudata ashmead hymenoptera.
Braconidae is one of the most important parasitoid species for the biological control of fruit flies. The diachasmimorpha longicaudata entomopoxvirus dlepv, the first reported symbiotic entomopoxvirus, occurs in the venom apparatus of d. It is a small genus relatively restricted to the subtropics but also includes species in the nearctic and northern neotropical regions. Braconidae is a larval endoparasitoid that has been research article. Braconidae and its interspecific competitive relationship with native fruit fly parasitoids in organic and conventional orchards in maceio, state of alagoas, brazil.
In order to make feasible the massrearing of the parasitoid diachasmimorpha longicaudata ashmed, this study investigated the suitability of a. The prominence of this species is attributed to its rapid adaptation to the media in which it is released, its great affinity with tephritidae, and the ease of rearing it in the laboratory garcia. The braconid diachasmimorpha longicaudata is the most widely used parasitoid in biological control programs of. Braconidae in citrus orchards in southeast brazil beatriz a. A new rodshaped nucleocapsids ncs was found inadvertently in diachasmimorpha longicaudata accessory gland filaments agfs. The caribbean fruit fly belongs to a group of major fruit pests that. Braconidae, potential parasitoids of the olive fruit.
Walder4 1brazilian agricultural research corporation embrapa tropical semiarid, laboratory of fruit flies, petrolina pe, brazil, 2cnpq, 3university of tessaly, department of agriculture. Braconidae, introduces an entomopoxvirus dlepv into its caribbean fruit fly host, anastrepha suspensa. Characterization of a venom glandassociated rhabdovirus in the parasitoid wasp diachasmimorpha longicaudata tyler j. Average weekly production of sterile anastrephaspp. The substrates pureed papaya or wheat diet in which treatment larvae were exposed to parasitoids did not affect oviposition preference of gravid d. Tephritidae in southern mexico john sivinski,1 kevina vulinec,2 and m. As the incidence of anastrepha fraterculus wiedemann has increased in southern brazil in the past 3 yr, an initiative to release sterile flies and parasitoids has started. Our study demonstrated the possibility that rearing in an insectary system may have modified the parasitization behavior of female d. Dispersion patterns of diachasmimorpha longicaudata.